SARS-CoV-2 Ubushyuhe buhoraho PCR Kumenya ibikoresho (Gukoresha murugo)
Ibisobanuro bigufi:
Kumenyekanisha ibicuruzwa ：
Ikoreshwa mugupima icyitegererezo cyo gupimwa fori idasanzwe ya acide coronavirus nucleic acide (ORF1ab, Ngene).
Ibiranga ibicuruzwa ：
• Byoroshye: Biroroshye gukora, kwiga, no gusobanukirwa, ntamahugurwa akomeye akenewe.
• Isothermal: Bika ikiguzi cyibikoresho.
• Umwihariko
guhitamo neza kugeza kuri 98%.
• Byihuse: Kumenya birashobora kurangira muminota 15.
• Gutwara no kubika neza: gutwara ubushyuhe bwicyumba no kubika, nta munyururu ukonje.
Ibicuruzwa byihariye ：
| Ikizamini / agasandukuTests Ibizamini 16 / agasanduku |
| ①swab②Swab yo kubungabunga tube③Amplification reaction tube④metal bath |
Ihame ry'ikizamini ：
Iki gikoresho cyerekana RNA ya SARS-CoV-2 ukoresheje uburyo bwo kongera imbaraga za isothermal.Guhindura transcription na mplification ya RNA ikorerwa mumiyoboro imwe.Urutonde rwa acide nucleic ya SARS-CoV-2 igaragazwa byumwihariko na primers esheshatu, kandi primer idahuye cyangwa idakorewe ntizuzuza amplification.Reagents zose hamwe na enzymes zisabwa kugirango reaction ibe yapakiwe mbere.Inzira yoroshye irakenewe kandi ibisubizo birashobora gukomoka kubireba ahari cyangwa fluorescence.
Fungura umufuka wa Aluminium hanyuma ukuremo imiyoboro ya reaction.Icyitonderwa, umuyoboro wa reaction ugomba gukoreshwa mugihe cyamasaha 2 umufuka wacyo wafunguwe.
Shyiramo ingufu.Igikoresho gitangira gushyuha (Ikimenyetso cyo gushyushya gihinduka umutuku kandi kimurika).Nyuma yo gushyushya, icyerekezo cyo gushyushya gihinduka icyatsi hamwe na beep.
Icyegeranyo cy'icyitegererezo ：
Shyira umutwe w'umurwayi inyuma ya 70 °, Reka umutwe wumurwayi uruhuke bisanzwe, hanyuma uzenguruke buhoro buhoro urukuta rwa ostril mu mazuru yumurwayi kugeza kumazuru, hanyuma ubikureho buhoro mugihe uhanagura.
Igisubizo cyiza: niba umuyoboro wa reaction ufite icyatsi kibisi cya fluorescence, ibisubizo nibyiza.Umurwayi akekwaho kuba yaranduye Sars-Cov-2.Hita hamagara umuganga cyangwa ishami ryubuzima ryaho hanyuma ukurikize amabwiriza yaho.
Igisubizo kibi: niba umuyoboro wa reaction udafite ibyatsi bya fluorescence bigaragara, ibisubizo nibibi.Komeza gukurikiza amategeko yose akurikizwa yerekeranye no guhura nabandi ningamba zo kubarinda.Hashobora no kwandura mugihe bipimishije nabi.
Igisubizo kitemewe: niba igihe cyo gukora kirenze min 20, amplification idasanzwe irashobora kubaho, biganisha kubintu byiza.Bizaba impfabusa hatitawe niba hari fluorescence igaragara, kandi ikizamini kizongera gukorwa.
