Malariya Pf / Pv Igikoresho cyo Kumenya Antigen

Yatugambiriye

Malariya ni indwara ikomeye, rimwe na rimwe yica, parasitike irangwa no kugira umuriro, gukonja, no kubura amaraso make kandi iterwa na parasite yandurira mu muntu ikajya mu rindi bitewe no kurumwa n'imibu ya Anopheles yanduye.Hariho ubwoko bune bwa malariya ishobora kwanduza abantu: Plasmodium falciparum, P. vivax, P. ovale, na P. malariya.Mu bantu, parasite (bita sporozoite) yimukira mu mwijima aho ikura ikarekura ubundi buryo, merozoite.Indwara nikibazo gikomeye cyubuzima muri tropike na subtropique.Abantu barenga miliyoni 200 kwisi barwaye malariya.

Kugeza ubu, malariya isuzumwa no gushakisha parasite mu gitonyanga cy'amaraso.Amaraso azashyirwa kumurongo wa microscope hanyuma uyisige irangi kugirango parasite zigaragare munsi ya microscope.Mugihe giheruka, ibibazo byo kwisuzumisha kwa clinique bijyanye na malariya ni ukumenya antibodiyite za malariya mumaraso yabantu cyangwa serumu na immunoassay.Imiterere ya ELISA nuburyo bwa immunochromatografique (byihuse) kugirango tumenye antibody ya malariya irahari vuba aha.

Ihame ry'ikizamini

Ikizamini cya Malariya Pf ni ikizamini cya immunochromatografique (byihuse) kugirango hamenyekane neza antibodies za isotypes zose (IgG, IgM, IgA) zihariye Plasmodium falciparum na vivax ya Plasmodium icyarimwe muri serumu yumuntu, plasma cyangwa amaraso yose.

Ibice nyamukuru

1. Ikarita yikizamini 2. Ikariso yinzoga ikoreshwa 3. Urushinge rwo gukusanya amaraso 4. Gukora
Imiterere yo kubika kandi ifite agaciro
1.Bika kuri 4 ℃ ～ 40 ℃ period igihe cyo kwemeza gishyirwaho mugihe cyamezi 24.
2.Nyuma yo gufungura umufuka wa aluminium, ikarita yikizamini igomba gukoreshwa vuba bishoboka muminota 30.Icyitegererezo cya diluent kigomba gufatwa ako kanya nyuma yo gufungura kigashyirwa ahantu hakonje.Nyamuneka koresha mugihe cyemewe.

Icyitegererezo

1. amaraso yose: Kusanya amaraso yose ukoresheje anti-coagulant.
2. serumu cyangwa plasma: Centrifuge maraso yose kugirango ubone plasma cyangwa serumu.
3. Niba ingero zidahita zipimwa zigomba gukonjeshwa kuri 2 ~ 8 ° C.Mugihe cyo kubika kirenze iminsi itatu, birasabwa gukonjesha.Bagomba kuzanwa mubushyuhe bwicyumba mbere yo gukoresha.
4. Ibigereranyo birimo imvura irashobora gutanga ibisubizo bidahuye.Ingero nkizo zigomba gusobanurwa mbere yo gusuzuma.
5. Amaraso yose arashobora gukoreshwa mugupima ako kanya cyangwa arashobora kubikwa kuri 2 ~ 8 ° C kugeza muminsi itatu.

Uburyo bwo Kwipimisha

Nyamuneka soma amabwiriza Amabwiriza yo gukoresha witonze mbere yo kwipimisha.Ingero zigomba gupimwa, reagent zerekana nibindi bikoresho bikoreshwa mugupimisha bigomba kuringanizwa nubushyuhe bwicyumba.Ikizamini kigomba gukorwa ku bushyuhe bwicyumba.
1.Kuraho ikarita yikizamini ushishimura umufuka wa aluminium, hanyuma urambike hejuru yimikorere.
2.Banza ukoreshe umuyoboro wa pulasitike kugirango wifuze igitonyanga 1 cyamaraso yose, serumu cyangwa plasma icyitegererezo (hafi 10μ1) mumariba yicyitegererezo (S) yikarita yikizamini.Noneho ongeramo ibitonyanga 2 kugeza kuri 3 (hafi 50 kugeza 100 μl) ya sample ya dilution
3.Kurikirana ibisubizo byubushakashatsi muminota 5-30 (ibisubizo ntibyemewe nyuma yiminota 30).
Icyitonderwa: Igihe cyo gusobanura hejuru gishingiye ku gusoma ibisubizo by'ibizamini ku bushyuhe bw'icyumba cya 15 ~ 30 ° C.Niba ubushyuhe bwicyumba cyawe buri munsi ya 15 ° C, noneho igihe cyo gusobanura kigomba kongerwa neza.

Gusobanura ibisubizo by'ibizamini

Ibyiza line Umurongo wamabara mukarere kayobora umurongo (C) ugaragara naho umurongo wamabara ugaragara mukarere kizamini （T）.Igisubizo ni cyiza.
Ibibi line Umurongo wamabara mukarere kayobora umurongo (C) ugaragara kandi ntamurongo wamabara ugaragara mukarere kizamini （T） .Ibisubizo nibibi.
Ntibyemewe ： Nta murongo ugaragara mu karere C.
Ntibyemewe ： Nta murongo ugaragara mu karere C.

Imipaka yuburyo bwo kugenzura

1. Ikizamini kigarukira gusa ku kumenya antibodies kuri Malariya zombi Plasmodium falciparum na vivax ya Plasmodium icyarimwe.Nubwo ikizamini ari ukuri cyane mugutahura antibodiyite kuri Malariya Pf, hashobora kubaho umubare muto wibisubizo byibinyoma.Ibindi bizamini biboneka mubuvuzi birakenewe niba habonetse ibisubizo bikemangwa.Kimwe n'ibizamini byose byo kwisuzumisha, isuzumabumenyi rishingiye ku mavuriro ntirigomba gushingira ku bisubizo by'ikizamini kimwe, ahubwo rigomba gukorwa na muganga gusa nyuma yo gusuzuma ibyavuye mu mavuriro na laboratoire.
2. Ibisubizo by'ibizamini by'iki gicuruzwa bisobanurwa n'amaso y'abantu, kandi birashobora kwibasirwa nibintu nk'amakosa yo kugenzura amashusho cyangwa guca imanza zifatika.Kubwibyo, birasabwa gusubiramo ikizamini mugihe ibara ryitsinda ritoroshye kumenya.
3. Iyi reagent ni reagent yujuje ubuziranenge.
4.Iyi reagent ikoreshwa mugutahura serumu yumuntu, plasma cyangwa amaraso yose.Ntukayikoreshe kugirango umenye amacandwe, inkari cyangwa andi mazi yo mumubiri

IBIKORWA BIKORWA

1. Ibyiyumvo byihariye kandi byihariye:Ikizamini cya Malariya Pf cyipimishije hamwe nubuvuzi bwiza kandi bubi bwapimwe na microscopique yo gusuzuma amaraso yose.
Malariya Pf ibisubizo byo gusuzuma

Ugereranije n'ikizamini cya Malariya Pf hamwe na microscopique yo gusuzuma amaraso yose, ibisubizo byatanze sensibilité ya 88.2% (150/170), umwihariko wa 98.5% (197/200), n'amasezerano yose hamwe 93.8% (347/370) .

2. Icyitonderwa
Mugihe cyo gukora neza byagenwe hakoreshejwe kopi 10 zingero enye zitandukanye zirimo antibody zitandukanye.Indangagaciro mbi kandi nziza zagaragaye neza 100% yigihe.
Hagati yo kwiruka neza byagenwe no gukoresha ingero enye zitandukanye zirimo ubunini butandukanye bwa antibody muri 3 itandukanye ikoporora hamwe nibikoresho 3 bitandukanye.Ubundi ibisubizo bibi kandi byiza byagaragaye 100% byigihe.

PROCAUTION

1. Kuberako muri vitro yo gusuzuma gusa.
2. Ntukarye cyangwa ngo unywe itabi mugihe ukoresha ingero.
3. Kwambara uturindantoki two kurinda mugihe ukoresha ingero.Karaba intoki neza nyuma.
4. Irinde kumeneka cyangwa gukora aerosol.
5. Sukura isuka neza ukoresheje imiti yica udukoko.
6. Kwanduza no kujugunya ingero zose, ibikoresho bya reaction hamwe nibikoresho bishobora kuba byanduye, nkaho ari imyanda yanduye, mubikoresho bya biohazard.
7. Ntukoreshe ibikoresho byo kwipimisha niba umufuka wangiritse cyangwa kashe yamenetse.

【Ironderero ryibimenyetso bya CE】